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產(chǎn)品中心產(chǎn)品培養(yǎng)基Gibco FreeStyle™ F17表達(dá)培養(yǎng)基
產(chǎn)品型號(hào)：
更新時(shí)間：2025-05-12
廠商性質(zhì)：代理商
訪問(wèn)量：1292
服務(wù)熱線
一、產(chǎn)品簡(jiǎn)介
產(chǎn)品中文名稱(chēng)：Gibco FreeStyle™ F17表達(dá)培養(yǎng)基
貨號(hào)：A1383501
產(chǎn)品描述：
Features:
• Little or no required adaptation
• Supports suspension growth and transfection of HEK293 cells
• Chemically defined, protein-free, serum-free formulation
• Compatible with multiple transient production systems
• Scalability in spinner flasks and bioreactors
Little or no required adaptation:
293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into FreeStyle F17 medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.
Growth and transfection of HEK293 cells:
FreeStyle F17 Expression Medium supports 293-F cells at a peak density of 6–7 x 106 viable cells per mL. Cells cultured in FreeStyle F17 medium can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin and Freestyle MAX Reagent.
Scalability in spinner flasks and bioreactors:
Production using FreeStyle F17 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement FreeStyle F17 medium with additional Pluronic F-68 to avoid sheer stress in the culture.
cGMP manufacturing and quality system:
FreeStyle F17 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
產(chǎn)品應(yīng)用：For research use only.
二、代理簡(jiǎn)介
Gibco FreeStyle™ F17表達(dá)培養(yǎng)基代理——北京百奧創(chuàng)新科技有限公司：
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3.基因組測(cè)序（二代，二代+三代）；
4.基因分型（SNP檢測(cè)）：低/中/中 高通量項(xiàng)目；
5.生物樣本運(yùn)輸（國(guó)內(nèi)、）；
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所有生物試劑耗材都可以進(jìn)口，特別是冷門(mén)品牌，均能給客戶購(gòu)買(mǎi)到。價(jià)格優(yōu)，貨期短，可加急，快10天到貨；保證服務(wù)質(zhì)量，所有產(chǎn)品均提供售后服務(wù)。大部分客戶我們提供貨到付款服務(wù)，
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專(zhuān)業(yè)訂購(gòu)國(guó)內(nèi)外試劑耗材，常備現(xiàn)貨，一手貨源，歡迎合作。
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